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General Discussions / News / Siderophore assays
Auteur Message
kieranw
posted on 12/10/2011 at 21:00 / Siderophore assays
Hi Everyone

Just wanted to let you know that I have made a test that can be used for detection of siderophores either in cell culture or in other biological fluids.The test has ready-to-use reagents,takes a couple of minutes to set up and will give you a result in 15 minutes.The test can be run in micortitre plates but also has potential for application on auto-analyzers, although we have not set this up on any system yet. However where you may have a lot of sample to analyze, it is feasible to run this test on a high though put analyzer.The test come available in a kit format with sufficient reagents for 100 tests, either for finding out if Siderophore is present (qualitiative applications) , or for determination of the amount of siderophore generated (quantitiatve application). A reference standard is provided in the kits for this.

We also use the test very successfully for assessing conjugation efficiency of siderophores to carrier proteins. In our case we used it for preparation of immunogens and for conjugation to other carrier materials.
If you need more information contact Kieran Walshe at kieran.walshe@nuim.ie or look at our web site for more detail www.emergenbio.com

Hope this is of some interest.

Kieran
Developed by Dr. Samuel Bertrand
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